Fitzgerald/p53 antibody/10R-1910/100 ul
商品编号：
10R-1910-100ul
品牌：
fitzgerald-fii
市场价：
¥6900.00
美元价：
5175.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
OVERVIEW:
| Synonyms | Monoclonal p53 antibody, Anti-p53 antibody, p53 Oncoprotein antibody, p53, p 53, p-53, p 53 antibody, p-53 antibody, BCC7 antibody, BOS_18908 antibody, brp53 antibody, cellular tumor antigen p53 antibody, drp53 antibody, EGK_08142 antibody, etID22686.5 antibody, fb40d06 antibody, LFS1 antibody, Li-Fraumeni syndrome antibody, p53 antibody, p53 tumor suppressor antibody, p53 tumor suppressor phosphoprotein antibody, phosphoprotein p53 antibody, transformation related protein 53 antibody, transformation-related protein 53 antibody, trp53 antibody |
|---|---|
| Cross Reactivity | Human |
| Applications | ELISA, IHC, WB |
| Immunogen | p53 antibody was raised in Mouse using a purified recombinant fragment of human p53 expressed in E. coli as the immunogen. |
SPECIFICATIONS:
| Host | Mouse |
|---|---|
| Clone | 4A8 |
| Isotype | IgG1 |
| Form & Buffer | Supplied as a liquid in ascitic fluid containing 0.03% sodium azide. |
| Concentration | 1.0 mg/ml |
STORAGE & SAFETY:
| Storage | Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
|---|
Usage Assay Information:
| Usage Recommendations | ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000 |
|---|
GENERAL INFORMATION:
| Product Use | Monoclonal p53 antibody specific for human p53 for use in ELISA, Immunohistochemistry and Western Blot |
|---|---|
| Biological Significance | p53 responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where its believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancerprone families with Li-Fraumeni syndrome. |
| Expression | High levels of p53 expression were found in many cancers; and the overexpression of an apparently wild-type TP53 gene transformed a normal cell into a cancer cell. P53 is widely dispersed in the cytoplasm, nucleus and endoplasmic reticulum. Localization is predominantly nuclear but translocates to the cytoplasm. Following cell stress, cytoplasmic p53 is directed to mitochondria where it interacts with pro- and anti-apoptotic members of the Bc12 family, resulting in the release of factors from the mitochondria that drive apoptosis. |
| Implications in Disease | Defects in p53 expression are a cause in several melanomas including lung cancer. If the TP53 gene is damaged, tumor suppression is severely reduced. People who inherit only one functional copy of the TP53 gene will most likely develop tumors in early adulthood, a disorder known as Li-Fraumeni syndrome. The TP53 gene can also be damaged in cells by mutagens (chemicals, radiation, or viruses), increasing the likelihood that the cell will begin decontrolled division. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. |
| Sequence Information | Member of the p53 family. Cytogenetic Location: 17p13.1 Molecular Location on chromosome 17: base pairs 7,668,401 to 7,687,549 |
| Post-Translational Modifications | p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. |
| Database Links | UniProtKB: P04637, Entrez Gene: 7157 Human, OMIM: 191170 Human, Unigene: 437460 Human |
品牌介绍
美国Fitzgerald 公司是一家国际性知名抗体研发生产企业。Fitzgerald公司为全球试剂诊断公司与研究机构提供专业的抗体、纯化抗原以及工业诊断用大包装免疫原料。公司产品多达5000种，能够满足您对于最前沿科学研究的需求。产品涉及：单克隆抗体／抗原生育类、传染病类、TORCH系列、肿瘤标记物、心脏标记物、神经科学类、病毒类等等。
品牌分类
重组蛋白
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