Fitzgerald/His Tag antibody/10R-7248/50 ul
商品编号：
10R-7248-50ul
品牌：
fitzgerald-fii
市场价：
¥1900.00
美元价：
1425.00
产品分类：
多克隆抗体
公司分类：
Polyclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
OVERVIEW:
| Synonyms | Monoclonal His Tag antibody, Anti-His Tag antibody, 6-His antibody, HHHHHH antibody, 6XHis antibody, HIS6 Tag antibody, Polyhistidine Tag antibody |
|---|---|
| Cross Reactivity | Human |
| Applications | ELISA, IF, IP, WB |
| Immunogen | His Tag antibody was raised in Mouse using 6x His synthetic peptide coupled to KLH as the immunogen. |
SPECIFICATIONS:
| Host | Mouse |
|---|---|
| Clone | 2A8 |
| Isotype | IgG1 |
| Method of Purification | His Tag antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in PBS buffer. |
STORAGE & SAFETY:
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.. |
|---|
Usage Assay Information:
| Usage Recommendations | ELISA: 1:2000, IF: 1:2000, IP: 1:100, WB: 1:5000 |
|---|
GENERAL INFORMATION:
| Biological Significance | Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. |
|---|
品牌介绍
美国Fitzgerald 公司是一家国际性知名抗体研发生产企业。Fitzgerald公司为全球试剂诊断公司与研究机构提供专业的抗体、纯化抗原以及工业诊断用大包装免疫原料。公司产品多达5000种，能够满足您对于最前沿科学研究的需求。产品涉及：单克隆抗体／抗原生育类、传染病类、TORCH系列、肿瘤标记物、心脏标记物、神经科学类、病毒类等等。
品牌分类
重组蛋白
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