OVERVIEW:
| Synonyms | MonoclonalFerritinHchainantibody,Anti-FerritinHchainantibody,FTH1antibody,ferritinantibody,heavypolypeptide1antibody,AL022624antibody,AL033366antibody,apoferritinantibody,BOS_24719antibody,cellproliferation-inducinggene15proteinantibody,ferritinHchainantibody,ferritinheavychainantibody,ferritinheavy-chainantibody,ferritinheavychain1antibody,ferritinHsubunitantibody,FerritinsubunitHantibody,fhcantibody,fthantibody,FTHL6antibody,H-ferritinantibody,HFtantibody,MFHantibody,OK/SW-cl.84antibody,pig15antibody,placentaimmunoregulatoryfactorantibody,PLIFantibody,proliferation-inducingprotein15antibody |
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| CrossReactivity | Human |
| Applications | ELISA,WB |
| Immunogen | FerritinHchainantibodywasraisedinMouseusingarecombinanthumanproteinpurifiedfromE.coli(HIS-FerritinHchain)astheimmunogen |
SPECIFICATIONS:
| Host | Mouse |
|---|---|
| Clone | 3F8 |
| Isotype | IgG2akappa |
| MethodofPurification | FerritinHchainantibodywaspurifiedbyammoniumsulfateprecipitation |
| Form&Buffer | SuppliedinliquidforminHEPESbufferwith0.15MNaCl,0.01%BSA,0.03%sodiumazide,and50%glycerol |
STORAGE&SAFETY:
| Storage | Aliquotandstorefor1yearat-20degC,avoidfreeze/thawcycles |
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UsageAssayInformation:
| UsageRecommendations | WB:1:1000 |
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GENERALINFORMATION:
| BIOLOGicalSignificance | ProductUses:MonoclonalFTH1antibodyspecificforhumanFTH1foruseinELISAandWesternBlotapplications. BiologicalSignificance:FTH1istheheavysubunitofferritin,themajorintracellularironstorageproteininprokaryotesandeukaryotes.Itiscomposedof24subunitsoftheheavyandlightferritinchains.Variationinferritinsubunitcompositionmayaffecttheratesofironuptakeandreleaseindifferenttissues.Amajorfunctionofferritinisthestorageofironinasolubleandnontoxicstate. Expression:FTH1expressionisregulatedbycellularironlevels. TissueandCellLocalization:FTH1isfoundinthemitochondrionandnucleusofmammaliancellswhereitisinvolvedincellularironiontransport. ImplicationsinDisease:Defectsinferritinproteinsmaybeassociatedwithseveralneurodegenerativediseases.DiseasesassociatedwithFTH1includehypertensiveretinopathy,aformofdamagetotheretinaandretinalcirculationduetohighbloodpressure.DefectsinFTH1mayalsoresultinironoverload,anaccumulationofironinthebody. SequenceInformation:FTH1iscomposedof183aminoacidswithamolecularweightof21226Da.Thereare2Phosphoserineresiduesatpositions449and553.Thefunctionalmoleculeformsaroughlysphericalshellwithadiameterof12nmandcontainsacentralcavityintowhichtheinsolublemineralironcoreisdeposited. Post-TranslationalModifications:UbiquitinationoccursatLys147. DatabaseReferences:EntrezGene:2495Human,Omim:134790Human,SwissProt:P02794Human,Unigene:524910Human |
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