OVERVIEW:
| Synonyms | MonoclonalACTA2antibody,Anti-ACTA2antibody,AAT6antibody,ACTAantibody,ACTA2antibody,actinalpha2smoothmuscleaortaantibody,Actinaorticsmoothmuscleantibody,ACTSAantibody,ACTVSantibody,alpha2actinantibody,Alpha-actin-2antibody,alpha-cardiacactinantibody,Cellgrowth-inhibitinggene46proteinantibody,growth-inhibitinggene46antibody |
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| CrossReactivity | Mouse,Rat,Monkey |
| Applications | ELISA,FC,IF,IHC,WB |
| Immunogen | ACTA2antibodywasraisedinMouseusingasynthesizedpeptideofhumanACTA2astheimmunogen |
SPECIFICATIONS:
| Host | Mouse |
|---|---|
| Clone | M72739 |
| Isotype | IgG1 |
| Form&Buffer | Suppliedinliquidformat,asciticfluidcontaining0.03%sodiumazideaddedaspreservative |
| Concentration | 1mg/ml |
STORAGE&SAFETY:
| Storage | Storeat4degCforshorttermstorage.Forlongertermstorage,aliquotandstoreat-20degC,avoidingrepeatedfreezethawcycles. |
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UsageAssayInformation:
| UsageRecommendations | WB:1-500-1-2000,IHC:1-200-1-1000,IF:1-200-1-1000,FC:1-200-1-400,ELISA:1-10000 |
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GENERALINFORMATION:
| BIOLOGicalSignificance | ProductUses:MonoclonalACTA2antibodyspecificforHumanACTA2foruseinELISA,Immunohistochemistry,FlowCytometryandWesternBlotapplications. BiologicalSignificance:Actinalpha2,thehumanaorticsmoothmuscleactingene,isoneofsixdifferentactinisoformswhichhavebeenidentified.Actinsarehighlyconservedproteinsthatareinvolvedincellmotility,structureandintegrity.Alphaactinsareamajorconstituentofthecontractileapparatus. Expression:SmoothmuscleACTA2expressionislargelyrestrictedtosmoothmusclecells,pericytesandspecializedfibroblasts. TissueandCellLocalization:Thisactinisanalphaactinthatismainlyfoundinskeletalmuscle. ImplicationsinDisease:ACTA2mutationspredisposepatientstoavarietyofdiffuseanddiversevasculardiseases,prematureonsetcoronaryarterydisease(CAD),themostcommontypeofheartdiseaseandcauseofheartattacks,causedbyplaquebuildingupalongtheinnerwallsofthearteriesoftheheart,prematureischemicstrokesandMoyamoyadisease,adiseaseinwhichcertainarteriesinthebrainareconstricted.Bloodflowisblockedbytheconstriction,andalsobybloodclots. SequenceInformation:ACTA2iscomposedof377aminoacidswithamolecularweightof42009Da,itBelongstotheactinfamily. Post-TranslationalModifications:ACTA2undergoesmonomethylationatLys-86(K84me1)whichregulatesactin-myosininteractionandactomyosin-dependentprocesses.DemethylationbyALKBH4isrequiredformaintainingactomyosindynamicssupportingnormalcleavagefurrowingressionduringcytokinesisandcellmigration.UbiquitinationoccursatLys52,Lys63,Lys70,Lys115,Lys193,Lys215,Lys293,Lys317,Lys328andLys330. DatabaseReferences:EntrezGene:59Human,Omim:102620Human,SwissProt:P62736Human,Unigene:500483Human |
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